This was not the first time that microbiologists experienced problems with agar. A phenomenon called “The Great Plate Count Anomaly” baffled researchers in the early 20th century when they observed that the number of cells seen under a microscope didn’t match the actual number of colonies growing on an agar plate. Investigating this discrepancy, researchers found agar itself to be the culprit: when nutrient broths are heated with agar during boiling, harmful byproducts (hydroperoxide) can form due to the reaction of agar with phosphate minerals contained in the media. Researchers can avoid this by autoclaving agar separately from the nutrient broth, or by reducing the amount of agar used.
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self.conn.close()，这一点在safew官方版本下载中也有详细论述
Link to Influence Flower
Kosmos 482, which launched in 1972 on a mission to Venus, never made it out of Earth's orbit and instead broke into four pieces that have been circling the planet for more than five decades.